ocelloscope detection system  (BioSense Solutions ApS)

Journal: Scientific Reports

Article Title: Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis

doi: 10.1038/s41598-019-50998-0

Figure Lengend Snippet: Diaphanoeca grandis . Number of individuals in filmed area in oCelloScope, grown on 3,000 (◇), 6,000 (○) and 12,000 (□) Pantoea sp. μL −1 . Inset. ln( D. grandis numbers) vs. time, exponential growth phase indicated by solid symbols, slope of regression line equals specific growth rates, µ (Supplementary Information, Table ).

Article Snippet: Growth of Diaphanoeca grandis was also monitored at the single-cell level in the oCelloScope optical detection system (Biosense Solutions Aps, Denmark), which is an automatic digital microscope equipped with a 4x optical magnification (200x effective magnification) , .

Techniques:

Journal: Scientific Reports

Article Title: Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis

doi: 10.1038/s41598-019-50998-0

Figure Lengend Snippet: Diaphanoeca grandis . Data on cohorts of individuals inoculated and filmed in the oCelloScope and grown on 3,000 (38 individuals, A – D ), 6,000 (40 individuals, E – H ) and 12,000 (40 individuals, I – L ) Pantoea sp. μL −1 . A , E and I . Number of cell divisions within 12 h time intervals. Solid bars indicate first release of a daughter cell, open bars indicate release of subsequent daughter cells. B , F and J . Number of inoculated D. grandis that have not yet carried out a cell division. C , G and K . Time span between cell divisions, excluding the first cell division (○), τ 1 and time span between replacement of a lorica and the following cell division (●). D , H and L . Number of protoplasts leaving and replacing their lorica within 12 h time intervals.

Article Snippet: Growth of Diaphanoeca grandis was also monitored at the single-cell level in the oCelloScope optical detection system (Biosense Solutions Aps, Denmark), which is an automatic digital microscope equipped with a 4x optical magnification (200x effective magnification) , .

Techniques:

Journal: Scientific Reports

Article Title: Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis

doi: 10.1038/s41598-019-50998-0

Figure Lengend Snippet: Diaphanoeca grandis . Characteristic life times (average values ± standard deviation) of individual cell stages from 3 cultures grown in the oCelloScope on Pantoea sp. (Figs 5 and 6 ). Time span between cell divisions, τ 1 . Life time of stage with two cells (mother and daughter) inside the parental lorica, τ 2 . Life time of motile, non-loricate cell stage, τ 3 . Time expenditure for assembly of new lorica, τ 4 . Time span since last cell division when a protoplast abandons its lorica, τ 5 . Number of events, n .

Article Snippet: Growth of Diaphanoeca grandis was also monitored at the single-cell level in the oCelloScope optical detection system (Biosense Solutions Aps, Denmark), which is an automatic digital microscope equipped with a 4x optical magnification (200x effective magnification) , .

Techniques: Standard Deviation

Journal: Scientific Reports

Article Title: Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis

doi: 10.1038/s41598-019-50998-0

Figure Lengend Snippet: Diaphanoeca grandis . Data on cohorts of individual D. grandis inoculated and filmed in the oCelloScope and grown on 3,000 (38 individuals, A – C ), 6,000 (40 individuals, D – F ) and 12,000 (40 individuals, G – I ) Pantoea sp. μL −1 . A , D and G . Life time of stage with two cells (mother and daughter) inside the parental lorica, τ 2 . B , E and H . Life time of motile, non-loricate cell stage, τ 3 . C , F and I . Time expenditure for assembly of new lorica, τ 4 . Open symbols indicate events following cell divisions. Closed symbols indicate events that occurred after a protoplast had abandoned its lorica.

Article Snippet: Growth of Diaphanoeca grandis was also monitored at the single-cell level in the oCelloScope optical detection system (Biosense Solutions Aps, Denmark), which is an automatic digital microscope equipped with a 4x optical magnification (200x effective magnification) , .

Techniques: